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P8670 人外周血白細胞分離液 solarbio 原裝
規格：200ml
保存：常溫

 P8670 人外周血白細胞分離液 solarbio 原裝 分離方法說明

A．取(qu)1ml新鮮(xian)抗凝血(xue)，與全血(xue)及組織稀釋液(ye)1:1 混勻后(hou)小心加于(yu)1份本分離液(ye)之液(ye)面上(shang)；

B. 以500g（約1800轉/分）離心25分鐘（半徑15cm水平轉子）。

此時離心(xin)(xin)管中由上下細(xi)胞(bao)(bao)(bao)分(fen)四層(ceng)(ceng)(ceng)。*層(ceng)(ceng)(ceng)；為(wei)(wei)(wei)(wei)血漿(jiang)層(ceng)(ceng)(ceng)。第(di)二層(ceng)(ceng)(ceng)；為(wei)(wei)(wei)(wei)環狀乳白(bai)色的(de)白(bai)細(xi)胞(bao)(bao)(bao)層(ceng)(ceng)(ceng)。第(di)三層(ceng)(ceng)(ceng)；為(wei)(wei)(wei)(wei)略帶混濁的(de)分(fen)離液(ye)(ye)層(ceng)(ceng)(ceng)（富集(ji)一定量的(de)白(bai)細(xi)胞(bao)(bao)(bao)）。第(di)四層(ceng)(ceng)(ceng)；為(wei)(wei)(wei)(wei)紅(hong)細(xi)胞(bao)(bao)(bao)層(ceng)(ceng)(ceng)。棄去(qu)*層(ceng)(ceng)(ceng)血漿(jiang)層(ceng)(ceng)(ceng)，收集(ji)第(di)二層(ceng)(ceng)(ceng)細(xi)胞(bao)(bao)(bao)、第(di)三層(ceng)(ceng)(ceng)分(fen)離液(ye)(ye)層(ceng)(ceng)(ceng)和第(di)四層(ceng)(ceng)(ceng)紅(hong)細(xi)胞(bao)(bao)(bao)層(ceng)(ceng)(ceng)，放入含(han)細(xi)胞(bao)(bao)(bao)洗滌液(ye)(ye)10ml的(de)試管中，充分(fen)混勻后，以500g約（1800轉/分(fen)）離心(xin)(xin)30分(fen)鐘。沉(chen)淀(dian)經1次洗滌后用紅(hong)細(xi)胞(bao)(bao)(bao)裂解(jie)液(ye)(ye)裂解(jie)紅(hong)細(xi)胞(bao)(bao)(bao)，再經3次洗滌去(qu)除紅(hong)細(xi)胞(bao)(bao)(bao)內(nei)溶物和碎片(pian)后取(qu)沉(chen)淀(dian)細(xi)胞(bao)(bao)(bao)即為(wei)(wei)(wei)(wei)所需白(bai)細(xi)胞(bao)(bao)(bao)

注： 提取率約(yue)為80%。