產品名稱：DMEM（H）（含雙抗，不含丙酮酸(suan)鈉） 培養基
產品型號：12100-500
產品報價：
產品特點：DMEM（H）（含雙抗，不含丙酮酸鈉） 培養基貨號：12100-046規格：10*1L英文名稱：DMEM, Powder, High Glucose儲存條件：2-8℃單位：瓶
免費(fei)咨詢(xun)：010-50973159
DMEM(H)(含雙抗，不含丙酮酸鈉) 培養基
貨號：12100-046
規格：10*1L
英文名稱：DMEM, Powder, High Glucose
儲存條件：2-8℃
單位：瓶
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
| With | Without |
| • High Glucose | • Sodium Pyruvate |
| • L-glutamine | • HEPES |
| • Phenol Red | • Sodium Bicarbonate |
The complete formulation is available.
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco® DMEM is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers a comparable Gibco® DMEM product made in our Scotland facility (52100-021). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
在(zai)中國境(jing)內, 本產品(pin)僅限于非臨床科(ke)研用途, 產品(pin)信息(xi)以中文為準(zhun)。 (In China territory, the product is for Research Use only in non-clinical unit, product information in Chinese shall prevail.)
儲存條件：2-8° C
運輸條件：環境溫度
保存期：自生產日期起36個月
DMEM(H)(含雙抗，不含丙酮酸鈉) 培養基
參考(kao)文(wen)獻：
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《A Host Factor GPNMB Restricts Porcine Circovirus Type 2 (PCV2) Replication and Interacts With PCV2 ORF5 Protein》 作者:Kangkang Guo, Lei Xu, Mengmeng Wu, Yufeng Hou, Yanfen Jiang, Jiangman Lv, Panpan Xu, Zhixin Fan, Ruiqi Zhang, Fushan Xing and Yanming Zhang 期刊:Frontiers in Microbiology 影響因子:4.019 PMID:30671053
《Effect of acute exposure to PFOA on mouse liver cells in vivo and in vitro》 作者:Xinmou Wu，Minqing Li，Zhao Yang，Min Su，Bin Yang 期刊:Environmental Science and Pollution Research 影響因子:2.8 PMID:28887612
《Trophoblast glycoprotein promotes pancreatic ductal adenocarcinoma cell metastasis through Wnt/planar cell polarity signaling》 作者:Ping He, Shuheng Jiang, Mingze Ma ,Yang Wang, Rongkun Li, Fang Fang, Guangang Tian ,Zhigang Zhang 期刊:Molecular Medicine Reports 影響因子:1.922 PMID:25738465
《High Expression of IL-4R Enhances Proliferation and Invasion of Hepatocellular Carcinoma Cells》 作者:C Guo, Y Ouyang, J Cai, L Xiong 期刊:The International Journal of Biological Markers 影響因子:1.449 PMID:28665449
《Effect of Mst1 overexpression on the growth of human hepatocellular carcinoma HepG2 cells and the sensitivity to cisplatin in vitro》 作者:C Xu, C Liu, W Huang, S Tu, F Wan 期刊:Acta Biochim Biophys Sin 影響因子:2.224 PMID:23419720
《TINAGL1 promotes hepatocellular carcinogenesis through the activation of TGF-β signaling-medicated VEGF expression》 作者:L Sun, Z Dong, H Gu, Z Guo, Z Yu 期刊:Cancer Management and Research 影響因子:3.702 PMID:30697069
《Selective anticancer activity of the novel steroidal dihydropyridine spirooxindoles against human esophageal EC109 cells》 作者:Xiaoli Shi,Tiantian Qin,Weihua Liu,Xueyan Zhang,Leilei Li,Junfeng Huo,Kairui Zhou,Dongxiao Yang,Yanling Zhang,Cong Wang 期刊:Biomedicine & Pharmacotherapy 影響因子:2.068 PMID:
《Gastroprotective effect of gamma-aminobutyric acid against ethanol-induced gastric mucosal injury》 作者:Min Xie,Haihong Chen,Shaoping Nie,Wei Tong,Junyi Yin,Mingyong Xie 期刊:Chemico-Biological Interactions 影響因子:3.296 PMID:28456544
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《Upregulated lncRNA small nucleolar RNA host gene 1 promotes 1‐methyl‐4‐phenylpyridinium ion‐induced cytotoxicity and reactive oxygen species production through miR‐15b‐5p/GSK3β axis in human dopaminergic SH‐SY5Y cells》 作者:Na Xie， Jinxing Qi， Shuang Li， Jianzhong Deng， Yuan Chen， Yajun Lian 期刊:Journal of Cellular Biochemistry 影響因子:3.448 PMID:30302821
《c-Myc induced upregulation of long non-coding RNA SNHG16 enhances progression and carcinogenesis in oral squamous cell carcinoma》 作者:Shangfeng Li, Shengkai Zhang & Jie Chen 期刊:Cancer Gene Therapy 影響因子:4.681 PMID:30607006
《Identification of Anticancer Compounds in Gallnuts Through PCA-constructed Secondary Metabolite Map》 作者:Jiayu Gao, Xiao Yang, Jiangxia Hu and Weiping Yin 期刊:International Journal of Pharmacology 影響因子:0.754 PMID:
《Label?free microfluidic chip for the identification of mesothelial cell clusters in pleural effusion》 作者:Lili Zhao, Meng Zhao, Yu Yang, Yajun Gu ,Fang Zheng ,Xuan Wang ,Zhiyuan Zheng, Xuguo Sun 期刊:Oncology Letters 影響因子:1.871 PMID:30944642
《Emodin Attenuates Lipopolysaccharide-Induced Injury via Down-Regulation of miR-223 in H9c2 Cells》 作者:Yuping Yang, Zijun Jiang, Dong Zhuge 期刊:International Heart Journal 影響因子:2.226 PMID:30745529
《miR?9 depletion suppresses the proliferation of osteosarcoma cells by targeting p16》 作者:Song Gao, Jianchao Wang, Shujian Tian, Jianping Luo 期刊:International Journal of Oncology 影響因子:3.571 PMID:31081054
《Long Non-Coding RNA NIFK-AS1 Augments the Oncogenicity of Hepatocellular Carcinoma by Regulating miR-497/BIRC5 Axis》 作者:Yajing Feng,Lu Sun,Yajuan Feng,Zhixian Guo 期刊:SSRN 影響因子: PMID:
《Sulfated modification enhanced the antioxidant activity of Mesona chinensis Benth polysaccharide and its protective effect on cellular oxidative stress》 作者:Lixin Huang,Min Huang,Mingyue Shen,Pingwei Wen,Ting Wu,Yuzhen Hong,Qiang Yu,Yi Chen,Jianhua Xie 期刊:International Journal of Biological Macromolecules 影響因子:4.784 PMID:31254574
《miR-185 silencing promotes the progression of atherosclerosis via targeting stromal interaction molecule 1》 作者:Ming Fang, Yanfei Li, Yingbiao Wu, Zhongping Ning, Xuejun Wang & Xinming Li 期刊:Cell Cycle 影響因子:3.259 PMID:30784343
《Insulin-like growth factor 2 mRNA binding protein 2 promotes aerobic glycolysis and cell proliferation in pancreatic ductal adenocarcinoma via stabilizing GLUT1 mRNA》 作者:Shan Huang , Zheng Wu , Yunyun Cheng , Wenzhen Wei Linlin Hao 期刊:Acta Biochimica et Biophysica Sinica 影響因子:2.502 PMID:31089713
《Dihydrotestosterone synthesis in the sheep corpus luteum and its potential mechanism in luteal regression》 作者:Longfei Xiao ， Liangli Song， Junjie Hu ， Quanwei Zhang ， Yuting Jiang ， Hongwei Duan ， Yong Zhang， Xingxu Zhao ， Yongli Hua 期刊:Journal of Cellular Physiology 影響因子:4.522 PMID:30671954
《Biological characteristics of heat shock protein 90 in human liver cancer cells》 作者:Li Qin, Haixin Huang, Jian Huang, Guodong Wang, Jinxin Huang, Xintian Wu, Jinzhuan Li, Weili Yi, Longjing Liu, and Dongning Huang 期刊:American Journal of Translational Research 影響因子:3.266 PMID:31105855
《Effect of Artesunate on the Expression of ICAM-1 and MMP-9 in Vascular Endothelial Cells under High Glucose Condition》 作者:Pengfei Ge, Tao Jiang, Yao Zong, Xuejiao Yang, Yuna Ma, Yunxiao Wang, Zhe Sun, Ziqun Cao 期刊:Hans Journal of Ophthalmology 影(ying)響因子:1.58 PMID:
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