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脫脂(zhi)奶粉（Westren Blot）是將鮮牛奶脫去脂肪再干燥而成，脂肪可降低1％左右，其他奶粉成份變化不大。

生產工藝：脫脂奶粉（Westren Blot）的生產工藝流程與全脂乳粉相類似，只是多了一個原料乳脫脂的過程。按熱處(chu)理(li)程度進行分類(lei)，分類(lei)指標(biao)為乳清蛋白氮指數(shu)（WPNI），乳粉中未變性乳清蛋白的(de)量(liang)與熱處(chu)理(li)程度成(cheng)反比。

低(di)熱(re)粉（WPNI>6mg/g乳粉）—72℃/15s或90℃/瞬時(shi)

中熱粉（1.5mg/g）

三、western blot中脫脂(zhi)奶(nai)粉的作(zuo)用

1，封(feng)閉(bi)

原(yuan)(yuan)理：用非抗原(yuan)(yuan)的蛋白去封閉膜(mo)的蛋白質結合位(wei)點，避免標記抗體固(gu)定在膜(mo)上引起(qi)背景增高，常用的封閉液(ye)有BSA,脫脂(zhi)奶粉,明膠, tween-20等,一(yi)般脫脂(zhi)奶粉。

實驗步驟：

5%脫(tuo)脂奶粉（用1x TBS配制(zhi)，或1xTBST配制(zhi)）封閉一(yi)晚上 或室溫1~2小時

2，一抗(kang)、二抗(kang)孵(fu)育

原理： 一(yi)抗(kang)可以與要(yao)檢測的(de)蛋(dan)白特(te)異結(jie)合，二抗(kang)可與一(yi)抗(kang)結(jie)合，同時二抗(kang)上帶(dai)(dai)有特(te)異的(de)酶（如辣根過氧化(hua)(hua)物(wu)酶HRP，堿性磷(lin)酸(suan)酶法AP等(deng) ),標記酶可以分解化(hua)(hua)學顯(xian)色底物(wu)產生有顏(yan)色的(de)條帶(dai)(dai)或催(cui)化(hua)(hua)化(hua)(hua)學發光底物(wu)使(shi)膠片感光。

實(shi)驗步驟：

一抗(kang)孵(fu)育(yu)：5%脫脂奶粉稀釋一抗(kang)到合適(shi)的(de)濃(nong)度(du)，與膜室溫孵(fu)育(yu)1小時或4度(du)一晚上（抗(kang)體濃(nong)度(du)及孵(fu)育(yu)時間需(xu)根(gen)據(ju)不同(tong)的(de)抗(kang)體做相應調整， 1xTBST，洗(xi)膜3次，每次5分鐘。

二抗孵(fu)育：5%脫(tuo)脂奶(nai)粉(fen)稀釋二抗到合適的濃度(du)，與膜室溫孵(fu)育1小時， 1x TBST,洗膜3次(ci)，每次(ci)5分鐘。

四、相關產品

C8070 Catalase   過氧(yang)化(hua)氫酶(mei)（牛肝）  1g

C8080 Cytochalasin B  細胞松弛素B  1mg

 C8650 Cyclophosphamide monohydrate環磷酰胺(an)  1g

C8100 DRISELASW 崩潰酶  500mg

C8110 ConA Type A IV 刀(dao)豆(dou)蛋白(bai)A IV  25mg

C8120 Cytochromes C  細胞(bao)色素C(馬心)  25mg

C8130 Cytochromes C  細胞色素(su)C（牛心）    25mg

TEVDAB-1000U TEV蛋白酶(mei) ;solarbio 1000u

C8140 CollagenaseⅠ  膠原酶Ⅰ  100mg

C8150 CollagenaseⅡ  膠原(yuan)酶Ⅱ  100mg

C8490 CollagenaseIII 膠原(yuan)III 進(jin)口分(fen)裝 100mg

C8160 CollagenaseⅣ  膠原酶Ⅳ  100mg

C8170 CollagenaseⅤ  膠(jiao)原(yuan)酶(mei)Ⅴ  100mg

相關文(wen)獻：

《Effect of 1α, 25-dihydroxyvitamin D3 on the osteogenic differentiation of human periodontal ligament stem cells and the underlying regulatory mechanism》 作者:Yawen Ji, Panpan Zhang ,Yixiao Xing ,Linglu Jia ,Yunpeng Zhang, Tingting Jia ,Xuan Wu ,Bin Zhao, Xin Xu 期刊:International Journal of Molecular Medicine 影響因子:2.928 PMID:30365053

五、公(gong)司簡介

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