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組織及血液堿性磷酸酶活性檢測試劑盒

AKP/ALP 是(shi)一種含(han)鋅(xin)的糖蛋白酶，在(zai)堿性(xing)環(huan)境中可水解各種天然及(ji)人(ren)工合(he)成(cheng)的磷脂單酯化合(he)物。AKP/ALP 廣泛(fan)分布于人(ren)體各臟(zang)器中，以肝臟(zang)為主。在(zai)堿性(xing)環(huan)境中，AKP/ALP 催化磷酸苯(ben)二鈉生(sheng)成(cheng)游離酚；酚與 4-氨(an)基安(an)替比林和鐵反應紅色亞醌(kun)衍(yan)生(sheng)物，在(zai) 510nm 有(you)特征光吸收；通過測定 510 nm 吸光度增加速率，來計算 AKP 活性(xing)。

組織及血液堿性磷酸酶活性檢測試劑盒

1.分光光度計預(yu)熱 30 min 以上(shang)，調節波長到 510 nm，蒸餾(liu)水調零。 2. 試劑二(er)置于 37℃水浴中(zhong)預(yu)熱 30 min 以上(shang)。試劑名稱（μL） 測定管(guan)(guan)(guan)(guan)(guan) 對照(zhao)管(guan)(guan)(guan)(guan)(guan) 空白(bai)管(guan)(guan)(guan)(guan)(guan) 標(biao)準(zhun)管(guan)(guan)(guan)(guan)(guan)蒸餾(liu)水 - - 4 - 2μmol/mL 標(biao)準(zhun)品 - - - 4 上(shang)清(qing)液(ye) 4 - - - 試劑一(yi) 40 40 40 40 試劑二(er) 40 40 40 40 混(hun)勻后置于 37℃水浴中(zhong)保溫 15min 試劑三(san) 120 120 120 120 上(shang)清(qing)液(ye) - 4 - - 混(hun)勻后于 510 nm 測定吸光度，分別記為 A 測定管(guan)(guan)(guan)(guan)(guan)、A 對照(zhao)管(guan)(guan)(guan)(guan)(guan)、A 空白(bai)管(guan)(guan)(guan)(guan)(guan)、A 標(biao)準(zhun)管(guan)(guan)(guan)(guan)(guan)

AKP/ALP 活(huo)(huo)性(xing)計(ji)算(suan)： 1.按蛋白(bai)濃度計(ji)算(suan)活(huo)(huo)性(xing)單(dan)位定(ding)(ding)(ding)(ding)義：37℃中每(mei)毫克(ke)蛋白(bai)每(mei)分(fen)鐘(zhong)催化產(chan)(chan)生(sheng) 1 μmol 酚(fen)。 AKP/ALP (U/mg pr)= [C 標(biao)(biao)(biao)準(zhun)(zhun)(zhun)品×(A 測定(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)(guan)-A 對(dui)照(zhao)管(guan)(guan)(guan)(guan)(guan))÷(A 標(biao)(biao)(biao)準(zhun)(zhun)(zhun)管(guan)(guan)(guan)(guan)(guan)-A 空(kong)(kong)(kong)白(bai)管(guan)(guan)(guan)(guan)(guan))×V 樣(yang)(yang)(yang)(yang)]÷(Cpr×V 樣(yang)(yang)(yang)(yang))÷T =0.133×(A 測定(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)(guan)-A 對(dui)照(zhao)管(guan)(guan)(guan)(guan)(guan))÷(A 標(biao)(biao)(biao)準(zhun)(zhun)(zhun)管(guan)(guan)(guan)(guan)(guan)-A 空(kong)(kong)(kong)白(bai)管(guan)(guan)(guan)(guan)(guan)) ÷Cpr 2.按樣(yang)(yang)(yang)(yang)本(ben)鮮(xian)重計(ji)算(suan)活(huo)(huo)性(xing)單(dan)位定(ding)(ding)(ding)(ding)義：37℃中每(mei)克(ke)組織每(mei)分(fen)鐘(zhong)催化產(chan)(chan)生(sheng) 1 μmol 酚(fen)。 ACP 活(huo)(huo)力(U/g)= [C 標(biao)(biao)(biao)準(zhun)(zhun)(zhun)品×(A 測定(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)(guan)-A 對(dui)照(zhao)管(guan)(guan)(guan)(guan)(guan))÷(A 標(biao)(biao)(biao)準(zhun)(zhun)(zhun)管(guan)(guan)(guan)(guan)(guan)-A 空(kong)(kong)(kong)白(bai)管(guan)(guan)(guan)(guan)(guan))×V 樣(yang)(yang)(yang)(yang)] ÷(W÷V 提(ti)取×V 樣(yang)(yang)(yang)(yang))÷T=0.133×(A 測定(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)(guan)-A 對(dui)照(zhao)管(guan)(guan)(guan)(guan)(guan))÷(A 標(biao)(biao)(biao)準(zhun)(zhun)(zhun)管(guan)(guan)(guan)(guan)(guan)-A 空(kong)(kong)(kong)白(bai)管(guan)(guan)(guan)(guan)(guan))÷W 3.血液中 ACP 活(huo)(huo)性(xing)計(ji)算(suan)活(huo)(huo)性(xing)單(dan)位定(ding)(ding)(ding)(ding)義：37℃中每(mei)毫升血液每(mei)分(fen)鐘(zhong)催化產(chan)(chan)生(sheng) 1μmol 酚(fen)。 ACP 活(huo)(huo)力(U)= [C 標(biao)(biao)(biao)準(zhun)(zhun)(zhun)品×(A 測定(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)(guan)-A 對(dui)照(zhao)管(guan)(guan)(guan)(guan)(guan))÷(A 標(biao)(biao)(biao)準(zhun)(zhun)(zhun)管(guan)(guan)(guan)(guan)(guan)-A 空(kong)(kong)(kong)白(bai)管(guan)(guan)(guan)(guan)(guan))×V 樣(yang)(yang)(yang)(yang)] ÷V 樣(yang)(yang)(yang)(yang)÷T =0.133×(A 測定(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)(guan)-A 對(dui)照(zhao)管(guan)(guan)(guan)(guan)(guan))÷(A 標(biao)(biao)(biao)準(zhun)(zhun)(zhun)管(guan)(guan)(guan)(guan)(guan)-A 空(kong)(kong)(kong)白(bai)管(guan)(guan)(guan)(guan)(guan)) C 標(biao)(biao)(biao)準(zhun)(zhun)(zhun)品：2μmol/mL；V 反(fan)總：反(fan)應(ying)體(ti)(ti)系總體(ti)(ti)積(ji)（mL），204μL=0.204mL； V 樣(yang)(yang)(yang)(yang)：加(jia)入(ru)反(fan)應(ying)體(ti)(ti)系中上清液體(ti)(ti)積(ji)（mL），0.004mL；T：反(fan)應(ying)時間（min），15 min；V 提(ti)取：加(jia)入(ru)提(ti)取液體(ti)(ti)積(ji)， 1mL；W：樣(yang)(yang)(yang)(yang)本(ben)鮮(xian)重，g。

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