產品名稱:anti-Flag Tag Mouse Monoclonal antibody(鼠單克(ke)隆抗體)
產品型號:M1001060
產品特點:anti-Flag Tag Mouse Monoclonal antibody(鼠單克(ke)隆抗(kang)體)在(zai)Western Blotting中(zhong)使用內(nei)參(can)其實就(jiu)是在(zai)WB過程中(zhong)的(de)另外用內(nei)參(can)對應的(de)抗(kang)體檢(jian)(jian)測(ce)內(nei)參(can),這樣(yang)在(zai)檢(jian)(jian)測(ce)目的(de)產物的(de)同時可以(yi)檢(jian)(jian)測(ce)內(nei)參(can)的(de)表達(da),由于內(nei)參(can)在(zai)各組(zu)織和細胞中(zhong)的(de)表達(da)相(xiang)對恒定(ding),借助檢(jian)(jian)測(ce)每個(ge)樣(yang)品(pin)內(nei)參(can)的(de)量就(jiu)可以(yi)用于校正上樣(yang)誤差,這樣(yang)半定(ding)量的(de)結果才(cai)更為可信。
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英文名稱 anti-Flag Tag Mouse Monoclonal antibody
應用 WB 1:2500 IP 1:100 IF:1:500 Optimal dilutions should be determined by the end user.
規 格 100ul
濃 度 1mg/ml
保(bao) 存(cun) Store at -20°C. Do not aliquot the antibody.
儲存液 PBS, pH 7.4, containing 0.02% sodium azide as Pretive and 50% Glycerol
純化方法(fa) affinity purified by Protein A
亞 型 IgG
性 狀 Liquid
克隆類型(xing) monoclonal
宿 主 Mouse
The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.
說明書(shu)
Anti- Flag Tag Mouse Monoclonal Antibody (1B3)
Catalog No. M1001060
Size:100μl
Storage: Store at -20°C. Do not aliquot the antibody.
The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to
interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively
as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to
separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can
also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many
different assays that require recognition by an antibody. If there is no antibody against the studied protein,
adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag
sequence.
Molecular Weight: N/A
Application: WB 1:2500 IP 1:100 IF:1:500 Optimal dilutions should be determined by the end user.
Specificity: The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag
fusion proteins.
Form: Liquid ,1mg/ml
Storage buffer: PBS, pH 7.4, containing 0.02% sodium azide as Pretive and 50% Glycerol
Relative Products
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M1001040 Anti-GST Tag Mouse Monoclonal Antibody(2C7)
M1001050 Anti- cMyc Tag Mouse Monoclonal Antibody(2C5)
M1001060 Anti- Flag Tag Mouse Monoclonal Antibody(1B3)
RG001010 Anti- HA Tag Rabbit Polyclonal Antibody
RG001020 Anti- His Tag Rabbit Polyclonal Antibody
RG001030 Anti- GFP Tag Rabbit Polyclonal Antibody
RG001040 Anti- GST Tag Rabbit Polyclonal Antibody
RG001050 Anti- cMyc Tag Rabbit Polyclonal Antibody
RG001060 Anti- Flag Tag Rabbit Polyclonal Antibody
Solarbio
Beijing Solarbio Science & Technology Co., Ltd
/82
Http://www.solarbio。。com
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