產品名稱:丙酮酸(suan)鈉 細(xi)胞培養試劑
產品型號:P8380
產品特點:丙酮酸鈉 細胞培養試劑貨號:P8380規格:25g/瓶CAS:113-24-6分子式:C3H3NaO3分子量:110.04儲存條件:2-8℃純度:≥99%外觀(性狀):白色粉末單位:瓶
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丙酮酸鈉 細胞培養試劑
貨號:P8380
規格:25g/瓶
測定乳酸脫氫酶的(de)底物; 細胞培養試(shi)劑(ji),用于補充碳源。
丙酮酸鈉是常見(jian)的丙(bing)酮酸(suan)鹽,是一(yi)類(lei)內(nei)源(yuan)性小分(fen)子物(wu)質,丙(bing)酮酸(suan)鈉(na)(na)和丙(bing)酮酸(suan)均是天(tian)然存(cun)在(zai)于人體(ti)內(nei),并參與全(quan)身各組(zu)織和器官的代謝。丙(bing)酮酸(suan)鈉(na)(na)在(zai)醫學(xue)上、診斷試劑以(yi)及(ji)醫療器械中(zhong)被廣泛用作(zuo)緩沖劑、賦形(xing)劑和抗氧化劑。此外丙(bing)酮酸(suan)鈉(na)(na)在(zai)細胞培(pei)養中(zhong)也是常用到(dao)的試劑之(zhi)一(yi),那么丙(bing)酮酸(suan)鈉(na)(na)在(zai)細胞培(pei)養中(zhong)的作(zuo)用是什么呢?
酮酸鈉在細胞(bao)培養中的作用(yong):
丙酮(tong)(tong)酸鈉(na)在(zai)(zai)人體(ti)日常生活(huo)中,是(shi)(shi)惹你進行(xing)代(dai)謝(xie)生命(ming)活(huo)動(dong)(dong)所必(bi)須(xu)(xu)的(de)(de)(de),它是(shi)(shi)一種能量(liang)物(wu)(wu)質(zhi)(zhi),在(zai)(zai)科學(xue)(xue)研究進行(xing)的(de)(de)(de)細(xi)(xi)胞培養試驗中,科學(xue)(xue)研究工作人員還發現(xian)了有作用(yong)類似(si)的(de)(de)(de)物(wu)(wu)質(zhi)(zhi),類似(si)參(can)與(yu)身體(ti)代(dai)謝(xie)活(huo)動(dong)(dong)的(de)(de)(de)為人體(ti)提供(gong)行(xing)動(dong)(dong)的(de)(de)(de)能量(liang)的(de)(de)(de)物(wu)(wu)質(zhi)(zhi)還有葡萄(tao)糖(tang),乳酸鈉(na)等,在(zai)(zai)人體(ti)中大多數(shu)細(xi)(xi)胞只利用(yong)葡萄(tao)糖(tang)就夠了,在(zai)(zai)細(xi)(xi)胞培養中丙酮(tong)(tong)酸鈉(na)是(shi)(shi)必(bi)須(xu)(xu)的(de)(de)(de)物(wu)(wu)質(zhi)(zhi)。丙酮(tong)(tong)酸鈉(na)在(zai)(zai)培養基中起到(dao)替代(dai)碳(tan)源(yuan)的(de)(de)(de)作用(yong),參(can)與(yu)細(xi)(xi)胞營養代(dai)謝(xie)。
丙酮酸鈉制備:
將領取(qu)的(de)丙(bing)酮酸(suan)在(zai)(zai)車間(jian)(jian)(jian)內(nei)放置一小(xiao)時(shi)以上,將抽(chou)(chou)料(liao)管用干(gan)(gan)(gan)凈(jing)的(de)聚酯纖維布包住管口(kou),緩(huan)緩(huan)插入桶底部(bu),用真(zhen)空(kong)(kong)將丙(bing)酮酸(suan) 280kg抽(chou)(chou)入丙(bing)酮酸(suan)鈉反(fan)應(ying)(ying)(ying)(ying)罐內(nei),抽(chou)(chou)畢(bi),開攪(jiao)拌,開攪(jiao)拌,將預(yu)處理好的(de)38~42℃碳酸(suan)鈉溶液(ye)緩(huan)緩(huan)壓反(fan)應(ying)(ying)(ying)(ying)罐內(nei)(控(kong)(kong)制(zhi)壓力在(zai)(zai)0.03~0.06MPa),控(kong)(kong)制(zhi)反(fan)應(ying)(ying)(ying)(ying)罐內(nei)溫(wen)(wen)度(du)不超過(guo)35℃(若反(fan)應(ying)(ying)(ying)(ying)過(guo)程中,溫(wen)(wen)度(du)超過(guo)35℃,應(ying)(ying)(ying)(ying)采(cai)取(qu)降溫(wen)(wen)措施),反(fan)應(ying)(ying)(ying)(ying)過(guo)程中應(ying)(ying)(ying)(ying)進(jin)行pH值檢(jian)測(ce),當反(fan)應(ying)(ying)(ying)(ying)液(ye)pH值達到4.8~5.3(用精密試紙測(ce)定)時(shi)停(ting)止加(jia)(jia)料(liao),控(kong)(kong)制(zhi)加(jia)(jia)料(liao)時(shi)間(jian)(jian)(jian)約1小(xiao)時(shi),反(fan)應(ying)(ying)(ying)(ying)完畢(bi),將1440kg 95%乙醇(chun)緩(huan)緩(huan)抽(chou)(chou)入(管口(kou)應(ying)(ying)(ying)(ying)用干(gan)(gan)(gan)凈(jing)的(de)聚酯纖維布包住)反(fan)應(ying)(ying)(ying)(ying)罐內(nei)(加(jia)(jia)入時(shi)速度(du)應(ying)(ying)(ying)(ying)先慢后快,開始(shi)50%的(de)量加(jia)(jia)入時(shi)間(jian)(jian)(jian)應(ying)(ying)(ying)(ying)控(kong)(kong)制(zhi)在(zai)(zai)60分(fen)(fen)鐘(zhong)內(nei),后50%的(de)量加(jia)(jia)入時(shi)間(jian)(jian)(jian)控(kong)(kong)制(zhi)在(zai)(zai)30分(fen)(fen)鐘(zhong)內(nei)),抽(chou)(chou)畢(bi),離心甩(shuai)干(gan)(gan)(gan),放入不銹鋼桶內(nei)送入烘(hong)房(fang),倒入雙錐真(zhen)空(kong)(kong)干(gan)(gan)(gan)燥箱內(nei),控(kong)(kong)制(zhi)真(zhen)空(kong)(kong)度(du)≥-0.09Mpa,熱水溫(wen)(wen)度(du)為75~80℃,真(zhen)空(kong)(kong)干(gan)(gan)(gan)燥器內(nei)溫(wen)(wen)上升為65℃時(shi),記錄(lu)起烘(hong)時(shi)間(jian)(jian)(jian),控(kong)(kong)制(zhi)真(zhen)空(kong)(kong)干(gan)(gan)(gan)燥器內(nei)溫(wen)(wen)度(du)為65~70℃,保溫(wen)(wen)2小(xiao)時(shi),自起烘(hong)2小(xiao)時(shi)后,請驗(yan),干(gan)(gan)(gan)失≤0.5%,冷(leng)卻約20分(fen)(fen)鐘(zhong),出料(liao),用20目過(guo)篩,包裝,入庫。收率應(ying)(ying)(ying)(ying)在(zai)(zai)105±5%之間(jian)(jian)(jian)。
丙酮酸鈉 細胞培養試劑
參考文獻:
《Simulated Cholinergic Reinnervation of β (INS-1) Cells: Antidiabetic Utility of Heterotypic Pseudoislets Containing β Cell and Cholinergic Cell》 作者:Ao Jiao, Feng Li, Chengshuo Zhang, Wu Lv, Baomin Chen, and Jialin Zhang 期刊:International Journal of Endocrinology 影響因子:2.287 PMID:
《Bufotalin induces cell cycle arrest and cell apoptosis in human malignant melanoma A375 cells》 作者:Zhaohai Pan,Chuanjun Qu,Ying Chen,Xiaoyu Chen,Xiaona Liu,Wenjin Hao,Wenjuan Xu,Lei Ye,Peng Lu,Defang Li,Qiusheng Zheng 期刊:Oncology Reports 影(ying)響(xiang)因子:3.041 PMID:30816542
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